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Cultrex 3D Culture Matrix Rat Collagen I
Cultrex 3D Culture Matrix Rat Collagen I from R&D Systems, a Bio-Techne Brand
Be the first to write a review!
Citations:
(29)
Supplier Page
Supplier Page from
R&D Systems, a Bio-Techne Brand for
Cultrex 3D Culture Matrix Rat Collagen I
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Cultrex 3D Culture Matrix Rat Collagen I
Company
R&D Systems, a Bio-Techne Brand
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
3447-020-01
Quantity
100 mg
Species
Rat
Molecule Name
Collagen I
Brand/Trademark
R&D Systems, a Bio-Techne Brand
Applications
Bioactivity
Format
Type I Collagen provided at 5 mg/ml (Sircol Assay)
NCBI Full Gene Name
collagen type I alpha 1 chain
NCBI Gene Aliases
CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4
Storage
Product is stable for a minimum of 3 months if stored at 4 °C.
Do Not Freeze.
Storage Buffer:
20 mM Acetic Acid
Sterility Testing:
No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP XXIV Chapter 71 sterility test. No mycoplasma contamination detected by PCR
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R&D Systems, a Bio-Techne Brand
614 McKinley Place N.E.
Minneapolis
,
Minnesota
55413
United States
Phone
: 1 (800) 343-7475
1 (612) 379-2956
Fax: 1 (612) 656-4400
Company Profile
Website:
http://www.rndsystems.com
Citations (29)
(1)
Development of a human iPSC-derived placental barrier-on-chip model
iScience
July 21, 2023
Agathe Lermant, Gwenaëlle Rabussier, Henriëtte L. Lanz, Lindsay Davidson, Iain M. Porter, Colin E. Murdoch
,
Tocris Bioscience
,Cat#2939 NaHCO3,
Sigma-Aldrich
,Cat#
S5761
Cultrex 3D Culture Matrix Rat Collagen I
,
R&D Systems
,Cat#
3447-020-01
"Fluorescein isothiocyanate-Dextran, 10 kDa",Sigma-Aldrich,Cat#FD10S "Tetramethylrhodamine isothiocyanate-Dextran,
(2)
Adipogenic and SWAT cells separate from a common progenitor in human brown and white adipose depots
Nature Metabolism
June 19, 2023
Nagendra P. Palani, Carla Horvath, Pascal N. Timshel, Pytrik Folkertsma, Alexander G. B. Grønning, Tora I. Henriksen, Lone Peijs, Verena H. Jensen, Wenfei Sun, Naja Z. Jespersen,
et al.
plate (5,000 cells per well). For adipogenic cells, the wells were pre-coated with rat collagen I (
3447-020-01
,
R&D systems
) for 1 h at 37 °C in a
cell culture
incubator to support adherence. Seahorse extracellular
(3)
Apabetalone Downregulates Fibrotic, Inflammatory and Calcific Processes in Renal Mesangial Cells and Patients with Renal Impairment
Biomedicines
June 8, 2023
Dean Gilham, Sylwia Wasiak, Brooke D. Rakai, Li Fu, Laura M. Tsujikawa, Christopher D. Sarsons, Agostina Carestia, Kenneth Lebioda, Jan O. Johansson, Michael Sweeney,
et al.
prepared on ice by combining 2.42 mg/mL
Cultrex rat collagen I
(
R&D Systems
, Minneapolis, MN, USA, #
3447-020-01
), 10× Minimum Essential Medium (MEM), and a solution of 0.05 N NaOH, 2.2% NaHCO3, and 200 mM
HEPES
pH
(4)
In vitro production of infectious Plasmodium falciparum sporozoites
Nature
December 1, 2022
Eappen AG, Li T, Marquette M, Chakravarty S, Kc N, Zanghi G, Hoffman BU, Hettiarachchi H, Patil A, Abebe Y,
et al
.
(5)
Analysis of reproducibility and robustness of OrganoPlate® 2-lane 96, a liver microphysiological system for studies of pharmacokinetics and toxicological assessment of drugs
Toxicology in Vitro
December 1, 2022
Yuki Kato, Alicia Y. Lim, Courtney Sakolish, Alan Valdiviezo, Haley L. Moyer, Philip Hewitt, Piyush Bajaj, Gang Han, Ivan Rusyn
(200 g, 3 min) and resuspended into 3.33 mg/mL collagen (Cultrex 3-D Culture Matrix Rat Collagen-I,
3447-020-01
,
R&D Systems
; 5 mg/mL type 1 collagen, 1 M
HEPES
, 37 g/L sodium bicarbonate at a ratio of 4:
1:1
, respectively)
(6)
Evaluation of Metabolism of a Defined Pesticide Mixture through Multiple In Vitro Liver Models
Toxics
September 27, 2022
Alan Valdiviezo, Yuki Kato, Erin S. Baker, Weihsueh A. Chiu, Ivan Rusyn
(200× g, 3 min) and resuspended into 3.33 mg/mL collagen (Cultrex 3-D Culture Matrix Rat Collagen-I,
3447-020-01
,
R&D Systems
; 5 mg/mL type 1 collagen, 1 M
HEPES
, 37 g/L sodium bicarbonate at a ratio of 4:
1:1
, respectively)
(7)
Nicotinamide‐N‐methyltransferase is a promising metabolic drug target for primary and metastatic clear cell renal cell carcinoma
Clinical and Translational Medicine
June 8, 2022
Anna Reustle et al.
minced into approximately 2 × 2 mm pieces and resuspended in type I collagen gel (
R&D Systems
Cat#
3447‐020‐01
). The tumour–collagen mixture was placed on top of a solidified collagen gel within a transwell insert
(8)
The transcription factor PAX8 promotes angiogenesis in ovarian cancer through interaction with SOX17
Science signaling
April 5, 2022
Chaves-Moreira D, Mitchell MA, Arruza C, Rawat P, Sidoli S, Nameki R, Reddy J, Corona RI, Afeyan LK, Klein IA,
et al
.
the 3D endothelial sprouting
assay
as previous described (60). Briefly, collagen type I (
R&D systems
:
3447-020-01
) was used as the 3D scaffold. After
incubation
for solidification, the plate was removed from the incubator
(9)
The aryl hydrocarbon receptor contributes to tissue adaptation of intestinal eosinophils in mice
The Journal of experimental medicine
April 4, 2022
Diny NL, Schonfeldova B, Shapiro M, Winder ML, Varsani-Brown S, Stockinger B.
37°C, 5% CO2.
Eosinophil
adhesion
assay
Plates were coated with 20 μl/96-well of
rat
collagen 1 (#
3447-020-01
;
R&D Systems
), Matrigel (Cultrex RGF BME Type II; #
3533-005-02
;
R&D Systems
), 0.1 mg/ml laminin (#
11243217001
;
(10)
Deficiency of lncRNA SNHG12 impairs ischemic limb neovascularization by altering an endothelial cell cycle pathway
JCI insight
January 11, 2022
Gross DA, Cheng HS, Zhuang R, McCoy MG, Pérez-Cremades D, Salyers Z, Wara AKMK, Haemmig S, Ryan TE, Feinberg MW.
(11)
Deoxynivalenol interferes with intestinal motility via injuring the contractility of enteric smooth muscle cells: A novel hazard to the gastrointestinal tract by environmental toxins
Ecotoxicology and environmental safety
August 16, 2021
Ji X, Qiao Y, Zheng W, Jiang H, Yao W.
(12)
Gamma-synuclein is a novel prognostic marker that promotes tumor cell migration in biliary tract carcinoma
Cancer medicine
August 1, 2021
Takemura Y, Ojima H, Oshima G, Shinoda M, Hasegawa Y, Kitago M, Yagi H, Abe Y, Hori S, Fujii-Nishimura Y,
et al
.
Plates (Essen BioScience) were coated overnight with 300 μg/ml of 3‐D Culture Matrix Collagen I (Cat#
3447‐020‐01
,
R&D systems
). The following day, collagen I dilution was aspirated, and cells were plated in six replicates
(13)
α-Catenin levels determine direction of YAP/TAZ response to autophagy perturbation
Nature communications
March 17, 2021
Pavel M, Park SJ, Frake RA, Son SM, Manni MM, Bento CF, Renna M, Ricketts T, Menzies FM, Tanasa R,
et al
.
(14)
3D spheroid models of paediatric SHH medulloblastoma mimic tumour biology, drug response and metastatic dissemination
Scientific reports
February 19, 2021
Roper SJ, Linke F, Scotting PJ, Coyle B.
(15)
Vav2 catalysis-dependent pathways contribute to skeletal muscle growth and metabolic homeostasis
Nature communications
November 16, 2020
Rodríguez-Fdez S, Lorenzo-Martín LF, Fernández-Pisonero I, Porteiro B, Veyrat-Durebex C, Beiroa D, Al-Massadi O, Abad A, Diéguez C, Coppari R,
et al
.
human
basic
fibroblast
growth factor (Cat. #hFGDBCF, Immunostep) on collagen type I-coated wells (Cat. #
3447-020-01
, Cultrex,
R&D Systems
). Activation and proliferation of satellite cells One thousand cells were plated
(16)
The Microfluidic Trainer: Design, Fabrication and Validation of a Tool for Testing and Improving Manual Skills
Micromachines
September 19, 2020
Costa F, Falzetti L, Baldini N, Avnet S.
(17)
Robust and Scalable Angiogenesis Assay of Perfused 3D Human iPSC-Derived Endothelium for Anti-Angiogenic Drug Screening
International journal of molecular sciences
July 7, 2020
van Duinen V, Stam W, Mulder E, Famili F, Reijerkerk A, Vulto P, Hankemeier T, van Zonneveld AJ.
Dulbecco’s phosphate-buffered saline
(dPBS, 14190-094, Thermo Scientific, USA), adjacent to a patterned collagen-1 gel (
3447-020-01
,
R&D systems
, UK). The ECs were cultured for 2 days in medium supplemented with 30 ng/mL vascular endothelial
(18)
SULF1 suppresses Wnt3A-driven growth of bone metastatic prostate cancer in perlecan-modified 3D cancer-stroma-macrophage triculture models
PloS one
January 1, 2020
Brasil da Costa FH, Lewis MS, Truong A, Carson DD, Farach-Carson MC.
consisting of hyaluronic acid (HA) (
Advanced BioMatrix
, CA;
GS220
),
3D culture
collagen I (Trevigen, MD;
3447-020-01
), and recombinant
human
perlecan domain I (PlnDmI), prepared as described in [70]. For each
Wnt3A
containing
(19)
Nrf2 and SQSTM1/p62 jointly contribute to mesenchymal transition and invasion in glioblastoma
Oncogene
December 1, 2019
Pölönen P, Jawahar Deen A, Leinonen HM, Jyrkkänen HK, Kuosmanen S, Mononen M, Jain A, Tuomainen T, Pasonen-Seppänen S, Hartikainen JM,
et al
.
(20)
A Role of Agrin in Maintaining the Stability of Vascular Endothelial Growth Factor Receptor-2 during Tumor Angiogenesis
Cell reports
July 23, 2019
Njah K, Chakraborty S, Qiu B, Arumugam S, Raju A, Pobbati AV, Lakshmanan M, Tergaonkar V, Thibault G, Wang X,
et al
.
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Cultrex 3D Culture Matrix Rat Collagen I from R&D Systems, a Bio-Techne Brand
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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